Outbreak Of Novel Coronavirus Pneumonia Research Articles

Relationship between health behavior compliance and prospect theory-based risk preferences during a pandemic of COVID-19

The outbreak of novel coronavirus pneumonia forced people to change their lifestyles. In this study, a questionnaire was administered to 1000 Chinese residents to investigate the effects of risk preferences based on behavioral economics prospect theory, trust and other people's expectations in a trust game, and personal attributes on the implementation of quarantine measures during an pandemic.Factor analysis divided the preventive behaviors into (1) compliance for self-health care, (2) compliance for going outside, and (3) compliance for staying home alone. In Category 1, those who were more sensitive to gains than losses were more likely to have a health code, wash their hands longer, report coronas, and disinfect often. In Category 2, they avoided volunteering at large hospitals, avoided trips to see acquaintances, avoided public walks, avoided meeting strangers, avoided going to garden centers, and wore masks when out and about. On the other hand, those more sensitive to loss than gain were found to work from home and socialize with non-family members in Category 3.Based on prospect theory, it was found that whether one values gains or losses has a strong influence on preventive behavior. In particular, the fact that those who value gains more than losses are more active in preventive behavior is expected to shed new light on future government guidance of citizens and nudges using nudges.

Rapid detection of SARS-CoV-2 spike protein using a magnetic-assisted electrochemical biosensor based on functionalized CoFe2O4 magnetic nanomaterials

The outbreak of novel coronavirus pneumonia (COVID-19) in 2019 has garnered widespread attention. The virus exhibits high contagiousness, and in certain cases, it can lead to recurrent infections. Therefore, it is imperative to develop portable, sensitive, and accurate sensors to promptly detect infected individuals, control the virus's transmission, and determine suitable treatment strategies. In this study, we proposed a magnetically-assisted method employing CFO@CS-Au MNP as the substrate material, which was functionalized with human angiotensin-converting enzyme (ACE2) for efficient capture of SARS-CoV-2 spike protein in solution. Subsequently, the captured protein was sensitively detected through differential pulse voltammetry (DPV) electrical analysis. The linear detection range of the labeled GCE/MNP/GA/ACE2/BSA electrochemical sensor is from 1 pg/mL to 10 μg/mL, with a minimum detection limit of 0.15 pg/mL. Furthermore, the fabricated GCE/MNP/GA/ACE2/BSA sensor achieved satisfactory recoveries of SARS-CoV-2 spike protein in saliva and nasal swab samples within 10 min. These results indicate that this magnetically-assisted biosensor has established a solid foundation for the swift on-site detection of COVID-19.

Polylevodopa nanoplatform for lateral flow immunochromatography assay of SARS-CoV-2 and influenza A virus

At the end of 2019, an unprecedented outbreak of novel coronavirus pneumonia ravaged the global landscape, inflicting profound harm upon society. Following numerous cycles of transmission, we find ourselves in an epoch where the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coexists alongside influenza viruses (Flu A). Swift and accurate diagnosis of SARS-CoV-2 and Flu A is imperative to stem the spread of these maladies and administer appropriate treatment. Presently, colloidal gold-based lateral flow immunoassays (Au-LFIAs) constructed through electrostatic adsorption are beset by challenges such as diminished sensitivity and feeble binding stability. In this context, we propose the adoption of black polylevodopa nanoparticles (PLDA NPs) featuring abundant carboxyl groups as labeling nanomaterials in LFIA to bolster the stability and sensitivity of SARS-CoV-2 antigens and influenza A virus identifications. The engineered PLDA-LFIAs exhibit the capacity to detect SARS-CoV-2 and Flu A within 30 min, boasting a detection threshold of 5 pg/ml for the SARS-CoV-2 antigen and 0.1 ng/ml for the Flu A H1N1 antigen, thereby underscoring their heightened sensitivity relative to Au-LFIAs. These PLDA-LFIAs hold promise for the early detection of SARS-CoV-2 and Flu A, underscoring the potential of PLDA NPs as a discerning labeling probe to heighten the sensitivity of LFIA across diverse applications.

[The non-bacterial pathogenic and clinical characteristics of acute respiratory tract infection in children in a hospital of pediatric in Sichuan Province from 2019 to 2021].

Objective: To explore the non-bacterial pathogen distribution, epidemiological characteristics, and clinical features of acute respiratory infections in children in Sichuan Province. Methods: Using a retrospective cohort study method, this study selected hospitalized children diagnosed with acute respiratory infections at West China Second Hospital of Sichuan University from February 2019 to January 2021, and tested 13 pathogens using polymerase chain reaction (PCR)-fragment analysis. The children were divided into infant group (<1 year old), toddler group (1 year old ≤ age <3 years old), preschool group (3 years old ≤ age <6 years old) and school-age group (6 years old ≤ age <18 years old). The distribution of pathogen positive rates, seasonal epidemic characteristics, clinical characteristics, and some laboratory test indicators were analyzed in children. Statistical analysis was performed on the results using SPSS 22.0 software, with count data expressed as percentages and inter group comparisons using SPSS 22.0 software χ2 Inspection. Results: A total of 2 922 pediatric patients were included in this study, with 1 748 (59.8%) positive for pathogens detected. Among them, 1 391 (79.6%) were detected as a single pathogen, and 357 (20.4%) were detected as a mixture of two or more pathogens. The most commonly detected pathogens were rhinovirus (HRV) (39.7%), syncytial virus (RSV) (22.8%), and parainfluenza virus (PIV) (12.5%). Pathogen positivity is more common in children under 6 years old (χ2=146.59, P<0.001), with a slightly higher positivity rate in male children (61.3%, 1 047/1 707) than in female children (57.7%, 701/1 215) (χ2=3.91, P=0.048), and compared with pathogen negative children, positive children are more prone to symptoms such as cough, wheezing, and shortness of breath (χ2=259.15, 366.06, 12.48, P<0.001). The distribution of different pathogens varies among children of different age groups, and HRV is more common in children aged 1-3 and 3-6 years old (χ2=9.74, P<0.001), while RSV is more common in children under 1 year old (χ2=178.63, P<0.001), while mycoplasma pneumoniae (MP) and influenza virus (InfA/B) are less common in children under 1 year old (χ2=92.54, 12.90,22.21, P<0.01). The prevalence of multiple pathogens showed seasonal changes. HRV showed a high prevalence trend in spring and autumn, while the prevalence of RSV infection was mainly seen in autumn and winter festivals. The positive rate of different pathogens after the outbreak of novel coronavirus pneumonia was significantly lower than that before the outbreak (χ2=252.68, P<0.001). Conclusion: The detection rate of non-bacterial respiratory pathogens in children in Sichuan Province from 2019 to 2021 is high, which is prone to symptoms such as cough, wheezing, and shortness of breath, with HRV and RSV being the main types. The positive rate of respiratory pathogens varies among different age groups, genders, and seasons.

Lipid Nanocarrier-Based mRNA Therapy: Challenges and Promise for Clinical Transformation.

Due to the outbreak of novel coronavirus pneumonia, messenger RNA (mRNA) technology has attracted heated attention. A specific, safe, and efficient mRNA delivery system is needed. Lipid nanocarriers have become attractive carriers for mRNA delivery due to their high delivery efficiency, few side effects, and easy modification to change their structures and functions. To achieve the desired biological effect, lipid nanocarriers must reach the designated location for effective drug delivery. Therefore, the effects of the composition of lipid nanocarriers on their key properties are briefly reviewed. In addition, the progress of smart drug delivery by changing the composition of lipid nanocarriers is summarized, and the importance of component design and structure is emphasized. Subsequently, this review summarizes the latest progress in lipid nanocarrier-based mRNA technology and provides corresponding strategies for its current challenges, putting forward valuable information for the future design of lipid nanocarriers and mRNA.

Analysis the Economic Recovery Strategies in China in the Post-Epidemic Era

The outbreak of novel coronavirus pneumonia has seriously damaged the global economy. Blockades and quarantine measures have caused the country's production activities to almost halt, supply chain disruptions, business closures, and reduced employment. Globally, nations have implemented monetary easing and fiscal stimulus measures to boost their economies. However, these measures may lead to inflation and debt crises. The tense geopolitical situation has exacerbated the dilemma of global governance. This article takes the post pandemic era as a research perspective and focuses on discussing complex economic measures. Taking the application strategies of countries during the Great Depression as an example, this study explores the relevant policies for China's economic recovery in the post pandemic era. The research results indicate that addressing economic recession requires a thoughtful combination of historical lessons and innovative solutions unique to contemporary challenges. The research results of this article have important practical significance for future economic development.

The mediation effect analysis of nurse's mental health status and burnout under COVID-19 epidemic.

The objective of this study is to investigate the mental health status of nurses during the outbreak of novel coronavirus pneumonia. Additionally, we aim to analyze the relationship between anxiety, depression, and burnout among nurses. The findings will provide a scientific basis for promoting the psychological health of nurses. Using a cross-sectional study, nurses in Quanzhou in May 2020 completed a general information questionnaire, the 7-item Generalized Anxiety Disorder Questionnaire (GAD-7), Patient Health Questionnaire-9 (PHQ-9), and the Maslach Burnout Inventory (MBI). Data analysis was conducted using structural equation model. 372 questionnaires were returned, with a response rate of 92.5%. The prevalence of anxiety and depression among the participants were 45.2 and 41.4%, respectively. The prevalence of severe burnout among nurses was found to be 7.3%. There was a correlation between nurses' anxiety, depression, and job burnout. The correlation coefficients between anxiety and job burnout, depression and job burnout, and anxiety and depression were found to be statistically significant (p < 0.001). Depression plays a mediating role between anxiety and jod burnout (0.584/1.413, 41.3%). The COVID-19 epidemic has resulted in moderate to high levels of job burnout among nurses. In this context, depression has been found to play a mediating role in the relationship between anxiety and job burnout. It is imperative for hospital administrators to prioritize the mental health of nurses and the provide necessary support to ensure their well-being.

A study on the factors influencing the development of the thinking and politics of higher education courses in China based on the “O2O” model

Abstract The outbreak of novel coronavirus pneumonia in early 2020 has attracted more and more attention to the O2O teaching mode to implement the deployment of “stopping classes without teaching and learning” during epidemic prevention and control. In this paper, we take the factors influencing the development of curriculum thinking politics in the continuing education of higher education as the object and use the Bayesian network to apply the influence indicators of curriculum thinking politics to complex scenarios for causal inference to explore the development path of curriculum thinking politics in the continuing education of higher education. According to the analysis of 1205 questionnaires of adult education, 49.95% of the students think that the concept of education has the problem of “understanding but not depth”, 61.41% of the students think that the content of curriculum thinking and political education has “shallow excavation but little integration”, and 51.96% of the students think that the teaching concept has “shallow excavation but little integration”. 51.96% of the participants think that the teaching method is “traditional and less interactive”, and 38.05% of the participants think that the evaluation of education is “professional but not political”, which are the four key factors that affect the development of the course of higher education in China. This paper proposes improvement methods through the four key factors of the development of the course of thinking and politics so that the students can receive knowledge and understand the ideological and political education at the same time and naturally build up their moral character, which can achieve the effect of “embellishment”.

Small molecule inhibitor CRT0066101 inhibits cytokine storm syndrome in a mouse model of lung injury

Pneumonia is an acute inflammation of the lungs induced by pathogenic microorganisms, immune damage, physical and chemical factors, and other factors, and the latest outbreak of novel coronavirus pneumonia is also an acute lung injury (ALI) induced by viral infection. However, there are currently no effective treatments for inflammatory cytokine storms in patients with ALI/acute respiratory distress syndrome (ARDS). Protein kinase D (PKD) is a highly active kinase that has been shown to be associated with the production of inflammatory cytokines. Therefore, small-molecule compounds that inhibit PKD may be potential drugs for the treatment of ALI/ARDS. In the present study, we evaluated the ability of the small-molecule inhibitor CRT0066101 to attenuate lipopolysaccharide (LPS)-induced inflammatory cytokine production through in vitro cell experiments and a mouse pneumonia model. We found that CRT0066101 significantly reduced the protein and mRNA levels of LPS-induced cytokines (e.g., IL-6, TNF-α, and IL-1β). CRT0066101 inhibited MyD88 and TLR4 expression and reduced NF-κB, ERK, and JNK phosphorylation. CRT0066101 also reduced NLRP3 activation, inhibited the assembly of the inflammasome complex, and attenuated inflammatory cell infiltration and lung tissue damage. Taken together, our data indicate that CRT0066101 exerts anti-inflammatory effects on LPS-induced inflammation through the TLR4/MyD88 signaling pathway, suggesting that CRT0066101 may have therapeutic value in acute lung injury and other MyD88-dependent inflammatory diseases.

A new autophagy-driven protective pathway of Haematococcus astaxanthin against chemical damage in wistar rats with gentamicin-induced acute kidney injury

Astaxanthin was established to conserve kidney function and subcellular structure through anti-oxidation and/or the free radical scavenging system, yet little research linked a new protective effect to autophagy or lysosomes. We pre-fed Wistar rats with natural astaxanthin, β-carotene, or placebo and induced acute kidney injury using gentamicin, before examining renal tissues and measuring physiological indices. Qualitative evidence from histopathological and subcellular images, along with quantitative evidence showing treatment effects on blood urea nitrogen and serum creatinine (p < 0.01), indicated that esterified Haematococcus astaxanthin surpassed β-carotene at effectively counteracting chemical damage and protecting the kidneys from injury. Proliferation of enlarged lysosomes and mediation analysis results revealing enhanced lysosomal acid phosphatase activity were consistent with the hypothesized autophagy-lysosomal pathway being up-regulated by astaxanthin intake (p < 0.05). In conclusion, the protective effect of astaxanthin against acute kidney injury exerted through the autophagy-lysosomal detoxification pathway, which totally different from the anti-oxidation and/or conventional SOD-dependent free radical scavenging system, was demonstrated with strong evidence. In light of the pandemic outbreak of novel coronavirus pneumonia associated with a virus preferentially targeting the renal tubular cells, dietary astaxanthin may help bring down incidence rate of coronavirus disease, cases of acute kidney injury secondary to the disease, and mortality rate from acute kidney injury, especially when a standard of care treatment for the infectious disease is pending.

Analysis on Seismic Performance of Prefabricated Building Box Formwork Combination

The outbreak of novel coronavirus pneumonia since the end of 2019 has posed a great challenge to the world's public health security. According to the statistics of COVID-19 epidemic released by the World Health Organization, the global epidemic has accumulated more than 500 million infections and more than 6.2 million deaths. However, if all hospitals at all levels are changed to designated hospitals, it may cause a run on medical resources. Container building has the advantages of simple construction, short construction period, recyclability and cost saving, and can be effectively used in the rapid transformation of talent apartment buildings and general wards into designated hospitals for efficient use. During the COVID-19, Wuhan Huoshen Mountain Hospital and Leishen Mountain Hospital adopted the container structure design, which is similar to Lego, combining containers one by one, giving full play to the advantages of container buildings. However, the current container building code stipulates that container buildings are only applicable to low and multi-storey buildings with six floors or less. In order to improve the seismic performance of container buildings and expand the application scope of container buildings, this paper proposes a box form composite structure based on traditional containers. Through the analysis of mechanical performance parameters, it is concluded that the "strong column and weak beam" yield mechanism of the box form composite structure is good, The initial rigidity of the weak axis is 133% higher than that of the traditional container structure, the yield strength is about 3 times that of the traditional container structure, and the ultimate bearing capacity is 2.5 times higher than that of the traditional container structure.

Prevalence, risk factors, and clinical correlates of anxiety, depression, and sleep disorders in chaperones for children in the emergency department in China during COVID-19.

The outbreak of novel coronavirus pneumonia in Wuhan, Hubei Province, in 2019 and its rapid spread across the country caused severe public panic in China. The purpose of this study was to investigate the mental health problems of children's chaperones at the emergency clinic during the coronavirus disease 2019 (COVID-19) outbreak and to analyze the related influencing factors. A total of 260 chaperones for children in the emergency department participated in this cross-sectional study through the questionnaire constellation platform. The survey period was from February to June 2021. Information collected included demographic data and mental health scales. The Self-Assessment Scale for Anxiety, the Self-Rating Scale for Depression, and the Pittsburgh Sleep Quality Index assessed anxiety, depression, and sleep quality, respectively. Logistic regression was used to analyze influential factors associated with mental health problems. The prevalence of depression, anxiety, and sleep disorders among family members accompanying children attending the emergency room was 41.54%, 20.00%, and 93.08%, respectively, with 21.54% of family members suffering from moderate sleep disorders. Univariate analysis showed that being in Wuhan or not during the city closure (X2 = 8.61, P < .01) was strongly associated with the occurrence of depression; female (X2 = 4.87, P = .03), working or not (X2 = 6.39, P = .01) and fear of going to the hospital (X2 = 7.80, P = .01) were key factors for the occurrence of anxiety symptoms; Knowledge of transmission routes and prevention of COVID-19 (X2 = 12.56, P = .03) was a key factor for sleep disorders; logistic stepwise regression analysis showed that fear of going to the hospital was a risk factor for anxiety symptoms (odds ratio = 2.51, P < .01, 95% confidence interval = 1.30-4.85). Our findings suggest that mental health problems were prevalent among family members accompanying children attending the emergency department during the COVID-19 outbreak, with a high prevalence of sleep disturbances in particular. Relevant factors included presence or absence in Wuhan during the outbreak closure, gender, work or absence, and fear of hospital visits. There is a need to focus on the mental health distress of the chaperones for children in the emergency department, and to provide timely intervention and diversion.

Study on college students’ willingness to inoculate COVID-19 vaccine based on bioinformatics and KAP analysis

ABSTRACT Bioinformatics is a subject that analyzes and interprets the collection, processing, storage and dissemination of biological information, and explores biological problems by using information technology and related means. The outbreak of novel coronavirus pneumonia has been nearly three years. With the advancement of vaccine research and development in various countries, mass vaccination is bound to usher in a new turning point for epidemic control. China’s policy of free vaccination for the new coronavirus vaccine will undoubtedly improve the vaccination rate, however, the vaccination rate still depends on people’s will. This paper takes college students as the main research object. In the context of bioinformatics analysis, a questionnaire survey was conducted based on their knowledge, attitudes, and practice levels of COVID-19 vaccines. The results vividly illustrate five conclusions. Firstly, the reliability of information sources positively affected college students’ cognitive level, degree of trust and inoculation intention of COVID-19 vaccination. Secondly, the professional degree of media positively affected college students’ degree of trust and inoculation intention behavior of COVID-19 vaccine. Thirdly, the cognitive level and degree of trust about COVID-19 vaccine positively affected college students’ intention behavior of COVID-19 vaccine vaccination. Fourthly, the positivity of information positively affected the willingness of college students to vaccinate against COVID-19 vaccine. Fifthly, there were significant differences in the willingness and behavior of college students with different genders in COVID-19 vaccination. Finally, three suggestions are given to improve college students’ willingness to vaccinate against the coronavirus and to cope with similar incidents in the future.

Standardized self-management education of psoriasis patients under New Coronavirus Pneumonia

Since the outbreak of novel coronavirus pneumonia (COVID-19) in December 2019, the epidemic has also had an adverse impact on the mental health of the public due to fear of the epidemic, common diseases, treatment delays and other factors. Patients with chronic skin diseases often have problems such as stress, anxiety, depression, anger and social isolation, which in turn will induce or aggravate primary skin diseases and form a vicious circle. Psoriasis is one of the common skin diseases. When the skin barrier function of patients with psoriasis is damaged, improper protection will increase the susceptibility to virus and induce or aggravate the disease. During the COVID-19 epidemic, some psoriasis patients who were isolated at home suffered from illness due to lack of disease awareness, poor self-management and lack. Patients who go out for medical treatment will increase the risk of COVID-19 infection. The lack of standardized self-management of psoriasis patients is one of the important reasons for the recurrence of their diseases. Standardized self-management is an important guarantee to improve the health status of patients and improve their quality of life. This article expounds the current situation and contents of standardized self-management of psoriasis patients, hoping to arouse medical workers' Thinking on how to implement standardized self-management of psoriasis patients under the epidemic situation of novel coronavirus pneumonia. At the same time, patients should strengthen self-management and do a good job in going out to prevent and minimize the adverse effects of the epidemic situation.

Research on innovative design of community micro space in post epidemic Era

In the case of novel coronavirus pneumonia, the strong spread of the virus caused certain psychological obstacles to outdoor activities. The community micro space is the main space for community residents to exercise and rest. In view of this phenomenon, it provides new methods and new ideas for exploring safe and healthy community interaction space design scheme, and achieves the purpose of controlling crowd aggregation through the division of space functions, so as to meet the needs of residents for the diversity of community space. After the outbreak of novel coronavirus pneumonia, it has become a multifaceted health care problem in the world, affecting the health of individuals and communities. In this case, "social distance" has become a major policy to restrict public behavior. This means that independent individuals must keep a proper safe distance from people other than their immediate family members, but cannot keep a distance from their immediate family members. In this context, people's demand for space security and diversity can no longer be satisfied by the traditional community public space. The traditional mode of community public space needs to be updated. This study provides a new method for the design and planning of community micro space, aiming to provide a new idea for the planning of community micro space, strengthen the interaction of community space by using mobile and modular outdoor facilities, ensure the safety of social streets, meet the diversified needs of community residents, create a community environment suitable for living for community residents, and provide a new planning for community residents.

A Study on the Relationship between the 3-D Structure of Spike Proteins and Infectiousness of SARS-CoV-2 Delta Variant

Since the outbreak of novel coronavirus pneumonia in Wuhan in 2019, the SARS-CoV-2 epidemic has become a hot topic. Over time, SARS-CoV-2 has evolved many variants. The diversity of the 3-D structure of the variant’s proteins resulted in the difference in the binding ability and infectious differences between different virus variants and human angiotensin-converting enzyme 2 (ACE2) receptors. In 2020, an evolutionary analysis of the Delta and Delta Plus variants of SARS-CoV-2 provided a three-dimensional model of the protein of the delta variant. However, it only focused on the delta variant and Delta plus variant themselves and did not compare the delta variant or delta plus variant with the original strain. It is hard to give a direct or apparent reason why the delta variant is more infectious and difficult to cure than the original strain. Therefore, this paper further compared the 3-D structures of homologous trimeric spike glycoproteins (S-proteins) and the receptor-binding domain between the SARS-COV-2 original strain and the SARS-COV-2 delta variant. By observing and analyzing the models of the above proteins in the PyMOL Molecular Graphics System, the reasons for the increase of infectivity of the delta variant can be interpreted in a direct way. This article also focuses on the data of the Indian cases from the JHU database to deeply analyze the relationship between the structure and transmission ability of the SARS-CoV-2 delta variant. Last but not least, the reproductive ability of SARS-CoV-2 can be reflected by the number of NAG (2-acetamido-2-deoxy-beta-D-glucopyranose). Through data analysis and protein structure research, we can better understand the characteristics of the binding of SARS-CoV-2 to the human receptor, thus providing a theoretical basis for accurately predicting virus variation. Through the comparative study of virus structure and infectiousness, this paper will provide a scientific basis for the relevant departments to improve epidemic prevention and improve the public's vigilance against virus variants.

Exploring the role of Xingren on COVID-19 based on network pharmacology and molecular docking.

Since the outbreak of novel Coronavirus Pneumonia 2019 (COVID‐19), the role of Almonds (Xingren) in the protection and treatment of COVID‐19 is not clear. Network pharmacology and molecular docking were used to explore the potential mechanism and potential key targets of Xingren on COVID‐19. A total of nine common targets between them were obtained, and these targets were involved in multiple related processes of GO and KEGG pathway enrichment analysis. Molecular docking showed that licochalcone B has the best binding energy (−9.33 kJ·mol−1) to PTGS2. They are maybe the important ingredient and key potential target. Its possible mechanism is to intervene anxiety disorder in the process of disease development, such as regulation of blood pressure, reactive oxygen species metabolic process, leishmaniasis peroxisome, and IL‐17 signaling pathway.Practical applicationsXingren is a traditional Chinese medicine that has been used and developed in China for many years. It contains a variety of active ingredients and also has the functions of relieving cough, relieving asthma, enhancing human immunity, delaying aging, regulating blood lipids, nourishing brain, and improving intelligence. In this article, the possible mechanisms of action and important targets of Xingren in the prevention and treatment of COVID‐19 were discussed through network pharmacology and molecular docking. We also found that active ingredient licochalcone B and the potential target PTGS2 are worthy of further research and analysis. At the same time, the study also provides a theoretical basis and reference for the prevention and treatment of COVID‐19 and the development of new drugs.

INFLUENCE OF LIFE VALUES EDUCATION ON COLLEGE STUDENTS WITH ANXIETY AND DEPRESSION DURING MAJOR EPIDEMIC

BackgroundThe outbreak of novel coronavirus pneumonia is a major public health event. The outbreak of this disease has a great impact on the whole society. After the outbreak of the epidemic, it has seriously affected the life values of college students and their understanding of life values. Some college students' cognitive bias has led to extreme events. From the perspective of development, life education group counseling is the need of people in contemporary society to pursue spiritual civilization, the need to implement and implement life education policies, and the need for individual self exploration and growth, which is conducive to promoting the development and progress of life education. In terms of theoretical significance, since the beginning of life education in China, there have been relatively few practical studies on life education, especially in the form of group psychological counseling. For life education in the early stage of development in China, this study enriches the forms of life education and provides a new research method for future life education research, Promote the further development of life education and the construction of related disciplines Set. From the perspective of practice, life education for depressed college students promotes the better development of life education in Colleges and universities, and provides systematic and targeted countermeasures for the development of life education courses in Colleges and universities. While promoting the development of College Students' life education, it will also point out the direction for the in-depth practice and research of College Students' life education. It is also conducive to the development of College Students' mental health, the maintenance of depressed college students' mental health and sound personality, and provide empirical basis and theoretical guidance for the development of College Students' mental health education, which is of great clinical significance.Subjects and MethodsAnalyze the impact of major epidemic on College Students' life values and the necessity of carrying out life values education; This paper introduces the PDCA cycle theory, introduces the connotation of PDCA cycle, and analyzes the feasibility of the application of PDCA cycle in the education of College Students' life values; Using PDCA cycle principle to carry out college students' life values education. SCL-90 scale was used to test the effect of students.ResultsDuring the major epidemic period, it is effective and feasible to use PDCA cycle to educate college students on life values, and solve the problems of life values faced by college students in different periods of 2019 coronavirus disease. PDCA cycle can constantly find and solve new problems and improve the effect of College Students' life values education.ConclusionPractice has proved that it is feasible to apply PDCA cycle to the education of College Students' life values, and the educational effect can be submitted. However, the application of PDCA cycle in college students' life values education has some limitations. We should accurately find the causes of the problems and formulate effective countermeasures. Cycle and time are not easy to control. This is also a problem to be solved in the later stage of PDCA cycle applied to college students' life values education. Therefore, we should: The premise of implementing life education is to establish a correct goal of life education, and pay attention to the two goals of instrumental and developmental life education. The goal of life education should not only prevent the occurrence of life damaging events, but also help students learn to protect life and improve their knowledge and skills of life survival while helping students understand the meaning of life and live in harmony with nature through scientific life education, Enhance the ability of life to withstand setbacks, so as to promote the better development of life. The goal of life education should also pay attention to starting from the perspective of students, vary from person to person, adhere to people-oriented, respect the law of students' development, and encourage students to creatively develop their own potential.AcknowledgementsThis paper is the phased achievements of the general planning project of Chongqing Education Science “13th Five Year Plan” in 2020 “Research on Dimensions and Implementable Strategies of College Students' Life Values Education Under The Background of Major Epidemic” (Project No.:2020-GX-416, Presider: Caixia Liao), Chongqing Higher Education Teaching Reform Research Project “Research and Practice on Improving Students' Innovation and Entrepreneurship Ability in Higher Vocational Colleges Under The Double High Plan" (Project No.:Z213203, Presider: Caixia Liao), 2021-2022 Higher Education Science Research Project of Chongqing Higher Education Society “Research on The Construction of Chengdu Chongqing Double City Economic Circle And The Coordinated Development of Higher Vocational Colleges” (Subject No.:CQGJ21B192, Presider: Caixia Liao), College Level Key Topics “Research on the path of Higher Vocational Colleges' Deep Participation in Rural Revitalization Strategy” (subject No.:2021XJKTZD02, Presider:Caixia Liao), Liao Caixia's innovation and entrepreneurship skills Master Studio of Chongqing Business Vocational College, Chongqing Institute of Business Economics, Chongqing Modern Service Industry Research Center, and Scientific Research and Innovation Team of Innovation and Entrepreneurship Education in Vocational Colleges.

XGBoost-Based Feature Learning Method for Mining COVID-19 Novel Diagnostic Markers.

In December 2019, an outbreak of novel coronavirus pneumonia spread over Wuhan, Hubei Province, China, which then developed into a significant global health public event, giving rise to substantial economic losses. We downloaded throat swab expression profiling data of COVID-19 positive and negative patients from the Gene Expression Omnibus (GEO) database to mine novel diagnostic biomarkers. XGBoost was used to construct the model and select feature genes. Subsequently, we constructed COVID-19 classifiers such as MARS, KNN, SVM, MIL, and RF using machine learning methods. We selected the KNN classifier with the optimal MCC value from these classifiers using the IFS method to identify 24 feature genes. Finally, we used principal component analysis to classify the samples and found that the 24 feature genes could effectively be used to classify COVID-19-positive and negative patients. Additionally, we analyzed the possible biological functions and signaling pathways in which the 24 feature genes were involved by GO and KEGG enrichment analyses. The results demonstrated that these feature genes were primarily enriched in biological functions such as viral transcription and viral gene expression and pathways such as Coronavirus disease-COVID-19. In summary, the 24 feature genes we identified were highly effective in classifying COVID-19 positive and negative patients, which could serve as novel markers for COVID-19.

Prognostic significance of inflammatory scoring systems in critically ill patients with COVID-19 infection

Aim: The outbreak of novel coronavirus pneumonia that was first detected in Wuhan in December 2019 resulted in a worldwide pandemic. Approximately 25% of confirmed cases developed a severe disease and the need for intensive care unit admission. The aim of this study was to determine the role of three inflammatory scoring systems, C-reactive protein/albumin ratio, fibrinogen/albumin ratio, C-reactive protein/lymphocyte ratio and their association with survival, comorbidities, and the occurrence of additional complications in the intensive care of these patients. Materials and methods: This retrospective study was conducted based on data collected by the Department of Respiratory Center. Ethical approval for the study was obtained from the Ethics Committee of Osijek University Hospital. This study analysed data retrospectively between October and December 2020, and included 137 critically ill patients with a diagnosis of COVID-19. Results: Data analysis of three examined inflammatory points of the system, below and above the median found a significant association in the group below the median C-reactive protein/albumin ratio with the presence of complications (p= 0,039) in the group above the median in the study of fibrinogen/albumin ratio found a significant association with sepsis (p=0,043). In the group of participants who were above its median in terms of C-reactive protein/lymphocyte ratio, there were more of those with the development of acute kidney injury (p=0,014), and sepsis (p=0,009). Conclusion: Inflammatory scoring systems, C-reactive protein/albumin ratio, fibrinogen/albumin ratio and C‐reactive protein/lymphocyte ratio represent an independent prognostic indicator of the clinical course in critically ill patients with COVID-19 infection.